Figure 7. (A) Histological scoring. The interleukin 10 gene-deficient (IL10^−/−) mice and the animals treated with a low dose of the zonulin peptide inhibitor AT-1001 had significantly increased histological scores as compared to the wild-type mice (*p<0.05). However, the group treated with a high dose of AT-1001 had a histological score significantly lower than the untreated IL10^−/− animals (**p<0.05), but this was still elevated significantly from the wild-type (WT) controls (n = 4–6). (B) Representative histology. All panels show colonic histology at 17 weeks of age. The upper panels (a, c, e, g) were taken at ×20 magnification, while the lower panels (b, d, f, h) are the same regions at ×40. Panels a/b represent tissue from the wild-type animals while panels c/d are from the IL10⁻/⁻ mice. A clear inflammatory infiltrate is observed in the lamina propria of these animals. Panels e/f are from the low-dose group while g/h are from the high-dose treated animals. It can be appreciated that there is a significant reduction in the inflammatory component of the lesion observed in these animals.