Figure 2.
Iron overload induced dose- and time-dependent increase in the levels of oxidative stress in RPE. A) Effect of different concentrations of FAC (4 days’ treatment) on the levels of intracellular oxidants. Changes in oxidative stress were measured with the oxidant-sensitive dye H2DCF. The significant increase in oxidant levels was induced by the concentrations of FAC of 250 µM or greater. The data represent mean ± sd of 6 samples from 3 different experiments (***P<0.001 vs. control). B) Time-dependent increase in the levels of intracellular oxidants in RPE cells treated with 250 µM FAC. The data represent mean ± sd of 6 samples from 3 different experiments (**P<0.01, ***P<0.001 vs. control).