Figure 4.
Effect of iron overload and NtBHA treatment on GSH levels in RPE cells. Control = untreated RPE cells; NtBHA = RPE cells treated with 250 µM NtBHA for 16 h; FAC, 4 days = RPE cells treated with 250 µM FAC for 4 days; FAC + 0 µM NtBHA = RPE cells treated with 250 µM FAC for 4 days and posttreated with NtBHA-free cell medium for 16 h; FAC + 250 µM NtBHA = RPE cells treated with 250 µM FAC for 4 days and posttreated with 250 µM NtBHA for 16 h. NtBHA treatment caused a significant increase in GSH levels in both control and FAC-treated RPE cells. The data represent mean ± sd of 5 samples from 3 different experiments (*P<0.05, ***P<0.001).