Figure 5.

Effect of iron overload and NtBHA treatment on ferritin and transferrin receptor (TfR) expression in RPE cells. A) Different treatments are indicated: Contr = control untreated RPE cells; NtBHA = RPE cells treated with 250 µM NtBHA for 16 h; FAC, 4 days = RPE cells treated with 250 µM FAC for 4 days; FAC + 0 µM NtBHA = RPE cells treated with 250 µM FAC for 4 days, then treated with NtBHA-free cell medium for 16 h; FAC = 250 µM NtBHA = RPE cells treated with 250 µM FAC for 4 days, then treated with 250 µM NtBHA for 16 h. B) Effect of NtBHA (250 µM, 16 h) treatment on ferritin levels in RPE cells from 3 different donors.