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Representative images of TUNEL staining of RPE nuclei. Fluorescein green signal indicates dUTP incorporation in apoptotic RPE nuclei (all RPE nuclei are counterstained with propidium iodide; red). (A) Control RPE cells; (B) RPE cells treated with t-BuOOH (C) RPE cells treated as in (B), but pretreated with 0.5 mM LA. Each micrograph is representative of RPE cultures from three different donors, with each experiment performed in duplicate.
