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Representative images of JC-1-labeled mitochondria. Loss of aggregate staining indicates a decrease in mitochondrial membrane potential. (A) Control RPE cells, (B) RPE cells treated with t-BuOOH, and (C) RPE cells treated as in (B) but pretreated with 0.5mMLA. Each panel is representative of three separate experiments, performed in duplicate and involving RPE cell cultures from three different donors.
