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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Arch Biochem Biophys. 2008 Oct 8;480(2):138–142. doi: 10.1016/j.abb.2008.10.001

Figure 1. Staurosporine induces mafA, but not insulin expression.

Figure 1

A, MIN6 cells were cultured in the presence of 25 mM or 1 mM glucose +/− 100 nM staurosporine overnight (16 to 18 hours) and MafA and TBP protein levels were analyzed by Western blotting (n > 3). B, mafA mRNA levels, in MIN6 cells cultured overnight on 25 mM, 1 mM, or 1 mM glucose plus 100 nM staurosporine, were analyzed by real-time RT-PCR (n=4). C, Western blot analysis of the stability of MafA and TBP in MIN6 cells incubated with 25 mM glucose +/− 40 μM cycloheximide (CHX), 1 mM glucose, and 1 mM glucose and 100 nM staurosporine +/− 40 μM cycloheximide (n > 4). D, Real-time RT-PCR analysis of insulin mRNA levels in MIN6 cells cultured in 25 mM, 1 mM, or 1 mM glucose plus 100 nM staurosporine overnight (n=4).