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. 2008 Dec 3;5(1):1–12. doi: 10.7150/ijbs.5.1

Figure 1.

Figure 1

Overall experimental design scheme to identify targets of BP1 in Hs578T breast cancer cells. ChIP-on-chip was performed on BP1-overexpressing Hs578T cell lines, O6 and O7. Specific target genes were identified by comparing with IgG-pulled DNA. Affymetrix Human Genome Focus arrays were used to identify differentially expressed genes associated with BP1 overexpression using BP1 overexpressors, O6 and O7 and empty vector controls, V1 and V2. Overlapping target genes between ChIP-on-chip and expression array analysis were identified for further analysis.