Table 3. Alternative RNA splicing in α-MHC-LacZ-(CTG)400 hearts.
Gene | Accession no. | Alt. spliced exon number | n | % Exons inclusion | |||||
WT adult | LacZ adult | TGhigh adult | Tglow adult | WT Posnatal day 1 | WT Posnatal day 2 | ||||
Tnnt2 | NM_011619 | 5 | 3 | 6.8±1.15 | 6.15±1.1 | 6.9±1.3 | 7.5±0.9 | 24.3±1.7* | 22.2±1.6* |
Alp | AF002283 | 5a | 3 | 81.7±2.0 | 80.2±1.6 | 80.5±1.8 | 81.1±2.5 | 98.2±0.5* | 97.7±1.0* |
Zasp | AY206013 | 11 | 3 | 67.9±4.7 | 65.5±3.1 | 67.6±4.1 | 69.1±5.3 | 91.6±0.7* | 89.7±1.3* |
m-Ttn | XM_130312 | Mex5 | 3 | 48.7±1.2 | 47.6±1.3 | 48.2±1.3 | 47.4±0.9 | 98.6±0.4* | 98.4±0.5* |
Asterisk (*) represents significant differences from wild type (WT) adult (Student's t-test; p<0.05). Tnnt2, cardiac troponin T; Alp, alpha-actinine-2 associated LIM protein; Zasp, Z-band alternatively spliced PDZ-motif protein; m-Ttn, M-line Titin; LacZ, α-MHC-LacZ-(CTG)0; TGhigh, α-MHC-LacZ-(CTG)400TGhigh; TGlow, α-MHC-LacZ-(CTG)400TGlow.