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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Anal Chem. 2008 May 9;80(13):5225–5231. doi: 10.1021/ac800553g

Figure 5.

Figure 5

Electrophoretic assay for RGS GTPase accelerating activity and its inhibition. (left) Basal hydrolysis by Gα alone results in formation of BGDP from BGTP; (middle) RGS protein accelerates the GTPase activity of Gα resulting in a larger BGDP peak formation; (right) addition of an inhibitor interferes with RGS and Gα interaction and thus diminishes the acceleratory effect of RGS protein resulting in a smaller BGDP peak in the electropherogram. All solutions were incubated for 20 min and then quenched with 10 μM GTPγS before electrophoretic analysis.