Skip to main content
. Author manuscript; available in PMC: 2008 Dec 9.
Published in final edited form as: Nat Neurosci. 2005 Jul;8(7):881–888. doi: 10.1038/nn1478

Figure 2.

Figure 2

DAT C-terminal residues 587-596 are sufficient to drive Tac endocytosis. (a) Schematic representation of the interleukin-2α receptor (Tac) and chimeric Tac-DAT proteins. (b) Single-cell Tac internalization assay. Antibody to Tac (anti-Tac) was bound at 4 °C to cells transfected with the indicated Tac-DAT cDNAs, and cells were warmed to 37 °C for 20 min. Internalized anti-Tac was detected with fluorescently labeled secondary antibodies using wide-field microscopy and image deconvolution. Internalization was scored in cases where Tac surface staining was lost, with a parallel appearance of intracellular puncta. Representative cells are shown. See Table 1 for summary of results.