Table 1. Single-cell Tac internalization assay.
Tac construct | n | Total number of cells imaged | Percentage of cells showing internalization |
---|---|---|---|
Tac | 26 | 77 | 2.3 ± 1.6 |
Tac-DAT 582-620* | 21 | 81 | 93.0 ± 2.7 |
Tac-DAT 596-620 | 7 | 27 | 18.3 ± 7.4 |
Tac-DAT Δ607-616* | 2 | 6 | 100.0 ± 0.0 |
Tac-DAT Δ596-616* | 2 | 4 | 100.0 ± 0.0 |
Tac-DAT Δ587-616* | 2 | 6 | 12.5 ± 12.5 |
Tac-DAT 587-596(10A) | 8 | 30 | 17.1 ± 7.1 |
Tac-DAT 587-591(5A)* | 6 | 28 | 60.0 ± 11.0 |
Tac-DAT 592-596(5A) | 5 | 16 | 15.0 ± 10.0 |
Tac-DAT L591A* | 3 | 17 | 83.3 ± 8.3 |
Tac-DAT Y593A* | 3 | 18 | 90.0 ± 9.1 |
Tac-DAT I595A | 10 | 43 | 12.4 ± 4.2 |
Significantly different from Tac, P < 0.01, one-way ANOVA with Dunnett’s multiple comparison test.