Table 2.
Efficiency of transformation into different sib deletion strainsa
vector | arabinose | MG1655 | ΔsibA | ΔsibC | ΔsibE |
---|---|---|---|---|---|
pAZ3 | − | 1.6 × 108 | 5.0 × 107 | 2.5 × 108 | 1.6 × 108 |
+ | 1.8 × 108 | 5.2 × 107 | 2.9 × 108 | 1.9 × 108 | |
| |||||
pAZ3-sibA | − | 7.8 × 106 | 3.6 × 107 | 2.6 × 106 | 6.0 × 105 |
+ | 8.8 × 106 | 2.6 × 107 | 4.1 × 106 | 5.0 × 105 | |
| |||||
pAZ3-sibC | − | 1.3 × 106 | 3.8 × 106 | no colonies | 6.0 × 105 |
+ | 1.3 × 106 | 2.1 × 106 | 1.0 × 107 | 1.5 × 106 | |
| |||||
pAZ3-sibE | − | 1.1 × 106 | 4.0 × 106 | 2.4 × 106 | no colonies |
+ | 2.7 × 106 | 2.2 × 106 | 3.8 × 106 | 2.0 × 106 |
Cells were transformed with 1 ng of DNA. Results similar to those observed for ΔsibC and ΔsibE were obtained for ΔsibB and ΔsibD.