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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Oct 21;876(1):69–75. doi: 10.1016/j.jchromb.2008.10.022

Table 1.

Results of previous studies examining binding of verapamil to HSA

Method (Conditions) Association Equilibrium Constant(s) (M−1) Reference
Capillary electrophoresis/frontal analysis (pH 7.4, 25°C) R-Verapamil, Ka = 2.67 (± 0.44) × 103 23a
S-Verapamil, Ka = 0.85 (± 0.11) × 103
Capillary electrophoresis/liquid pre-column (pH 7.4, 25°C) R-Verapamil, Ka = 2.7 (± 0.44) × 103 21a
S-Verapamil, Ka = 0.84 (± 0.16) × 103
Capillary electrophoresis/frontal analysis (pH 7.4, 25°C) Racemic verapamil, Ka =1.69 (± 0.13) × 103 to 1.79 (± 0.70) × 103 22,24b
Capillary electrophoresis/frontal analysis (pH 7.4, 25°C) Racemic verapamil, Ka = 1.10 (± 0.03) × 103 28
Equilibrium dialysis (pH 7.4, 37°C) Racemic verapamil, Ka= 1.16 × 105 and 6 × 103 29
a

The same authors working with apparently the same system report in Ref. 19 an essentially identical value for R-verapamil but Ka = 8.5 (± 0.1) × 103 M−1 for S-verapamil; this appears to be the result of a typographical error.

b

This range of values was obtained by using several data analysis methods for the same set of experimental results.