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Figure 2 Results of semiquantitative determination of differentially methylated region methylation by duplex polymerase chain reaction (PCR) in (A) patients and (B) controls. Methylated (meth, 167 bp) and unmethylated (umeth, 226 bp) alleles. (C) Comparison of methylation results obtained by bisulphite sequencing and duplex PCR. L, methylation loss; pL, partial methylation loss; x, contradictory results resolved by finding normal methylation in the B2 region.
