Table 2 Nucleotide sequences of the MPLA probes for detection of GLDC deletions.
| Target gene | Probe name | Nucleotide sequences (5′ to 3′) |
|---|---|---|
| GLDC | M‐GLDC‐E1U | GGGTTCCCTAAGGGTTGGAGAGAGAGATGCTGCAGACCTTGGGGCTGGCG |
| M‐GLDC‐E1D | *GTAAGGACCTCCACCCGGCCCTCCGCGTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E2U | GGGTTCCCTAAGGGTTGGATTTGAAAAGACCCTTGAAAATGGAAGACCCTGTTT | |
| M‐GLDC‐E2D | *GTAAGTGGCCGGGAGGGCTCCCTTGGACTTATCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E3U | GGGTTCCCTAAGGGTTGGAACAGACGATTTTGCGGAACTTACTGGAGAACTCAGGATG | |
| M‐GLDC‐E3D | *GTAATGTATTTCTCAGTTCAGGAACAGGATGACTGTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E4U | GGGTTCCCTAAGGGTTGGAATGAGGGGACTGCAGCCGCAGAGGCACTGCAGCTGTGCTACAG | |
| M‐GLDC‐E4D | *GTGAGAGGCCTCTCAAAGTGCTGGAATTCCAGTTGTGGGTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E5U‐2 | GGGTTCCCTAAGGGTTGGACTATTTATTTAATGTTCACGTTGGAATGTGCTTTTTCTTTTCAACAG | |
| M‐GLDC‐E5D‐2 | *ACACAACAAGAGGAGGAAATTTCTCGTTGATCCCCGTTGCCACTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E6U | GGGTTCCCTAAGGGTTGGAGGGAAGGTGGAAGACTTTACGGAACTCGTGGAGAGAGCTCATCAGAGTGGG | |
| M‐GLDC‐E6D | *GTAGGTATACCTTTCTTGTGGGGGGTCCGTGGAGGCGTATCCCAACTTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E7U | GGGTTCCCTAAGGGTTGGACTGTCCGAGAAAGCTTGGTGAGAATGATGCCTGGAAGAATGGTGGGGGTAACAAG | |
| M‐GLDC‐E7D | *GTAAAGGGGCTCATGTTTCTCTACTTTTATTGTGATTATGATTTCCCTGATTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E8U‐2 | GGGTTCCCTAAGGGTTGGACCATTTTCTCAGTGGGAACTAAGGGCGGGCCTCTTCAGTTCCCAC | |
| M‐GLDC‐E8D‐2 | *CTGAGCATTCATATTTGCCCCGTCTAGGTAGACCTGTCCTCTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E9U | GGGTTCCCTAAGGGTTGGAATGTTCCCATGGGCTGGAGCATATTGCTAGGAGGGTACATAATGCCACTTTGATTTTGTCAGAAG | |
| M‐GLDC‐E9D | *GTGAGTTGGTAATCTGTCTAAAACATTTGGGCATAATAAAATTGATAAATTTGAGTATCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E10U | GGGTTCCCTAAGGGTTGGAGCTGCTAGTGAAGGAGGTCTTGGGCAGGGCCGCTCAGCGGCAGATCAATTTTCGGCTTTTTGAGGATGGCACA | |
| M‐GLDC‐E10D | *GTAAGTCAAATTTTCAGTATTTTTACCAGTTTTTCAAATTTTCACATTGTTTCTCATTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E11U | GGGTTCCCTAAGGGTTGGACTTGGTATTTCTCTTGATGAaACAGTCAATGAAAAAGATCTGGACGATTTGTTGTGGATCTTTGGTTGTGAGTCATCTGCA | |
| M‐GLDC‐E11D | *GTAAGTAAAATAAAAACATGCGTTCCTCAtCATAACTATTGGAGGTGGTAGCAAAAGTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E12U | GGGTTCCCTAAGGGTTGGAGCTGAAAGCATGGGAGAGGAGTGCAGAGGTATTCCAGGGTCTGTGTTCAAGAGGACCAGCCCGTTCCTCACCCATCAAGTGTTCAACAG | |
| M‐GLDC‐E12D | *GTTTGTGTGTCTTGTGTGACTTCTGCGTTTTGTGCTTTGGTAATCAGCTAGGGAGTTTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E13U‐2 | GGGTTCCCTAAGGGTTGGATGTTCACAGCTACCACTCTGAAACAAACATTGTCCGGTACATGAAGAAACTGGAAAATAAAGACATTTCCCTTGTTCACAGCATGATTCCACT | |
| M‐GLDC‐E13D | *GGTAGTTATTTGTGGCCTTTTTTCTCATTTCCAAGCTACCCCAATCCCACGTCTCTTTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E15U | GGGTTCCCTAAGGGTTGGAAGGTTATGACCAGGTCTGTTTCCAGCCAAACAG | |
| M‐GLDC‐E15D | *GTAAGGGCATTTCTTTTCTTATTGTTTCATCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E16U | GGGTTCCCTAAGGGTTGGAAGCCTACTTAAACCAGAAAGGAGAGGGGCACAGAACG | |
| M‐GLDC‐E16D | *GTGAGTATGGCAGGAGGTGGCGCTTGCTCACCATCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E17U | GGGTTCCCTAAGGGTTGGAATAAATATGGGAATATCGATGCAGTTCACCTCAAGGCCATG | |
| M‐GLDC‐E17D | *GTACTTGTCTTCTCCTTAGCAGATGGGAGAGGCCGGATCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E18U‐2 | GGGTTCCCTAAGGGTTGGACCATTTTCTCAGTGGGAACTAAGGGCGGGCCTCTTCAGTTCCCAC | |
| M‐GLDC‐E18D‐2 | *CTGAGCATTCATATTTGCCCCGTCTAGGTAGACCTGTCCTCTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E19U | GGGTTCCCTAAGGGTTGGATCTGCATTCCCCACGGAGGAGGTGGTCCTGGCATGGGGCCCATCGGAGT | |
| M‐GLDC‐E19D | *GTAAGTTCTGGGCTGCTGGTTTCAGGATGGCTTTGGAGACAGAATTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E20U | GGGTTCCCTAAGGGTTGGACGGCCCCATGGGGCTCCAGTTCCATCTTGCCCATTTCCTGGGCTTATATCAAG | |
| M‐GLDC‐E20D | *GTGAGGCCTGGGAGTATGTGCAGGTGTGCAGGTGGGTGGGGGCGTCAGGTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E21U | GGGTTCCCTAAGGGTTGGAACTACATGGCCAAGCGATTAGAAACACACTACAGAATTCTTTTCAGGGGTGCAAGAG | |
| M‐GLDC‐E21D | *GCAAGTATCAACTTTAATCTATCATTACTTGGTTTTTTTCTTGGCCAAACTAATCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E22U | GGGTTCCCTAAGGGTTGGACCCTTCAAAAAGTCTGCAAATATTGAGGCTGTGGATGTGGCCAAGAGACTCCAGGATTATG | |
| M‐GLDC‐E22D | *GTAAGTGGCTTTTGACATTCATGCCGCCGCCCATGCTGGCTGTGGACCACTTCCTAATCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E23U | GGGTTCCCTAAGGGTTGGAATCAGCATTCGGCAGGAAATTGCTGACATTGAGGAGGGCCGCATCGACCCCAGGGTCAATCCGCTGAAG | |
| M‐GLDC‐E23D | *GTGCGTAGGCCCTGGAACATTGCTTGAAATGTTCCTTAAACTAGAAAATGATGTCTGTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E24U‐2 | GGGTTCCCTAAGGGTTGGAGCTAAGAGCGTACACCCGTCAGGATAGGAGCTGGCCCATGCCTTCCCAGCTGGCACATTCAGATTCAGAGAACTTAC | |
| M‐GLDC‐E24D‐2 | *GAGTGGGAATGCTGCCACCTCTCTGGAATAAGGCCGGTCCCAGTGGGAAGATGTAACTCTAGATTGGATCTTGCTGGCAC | |
| M‐GLDC‐E25U | GGGTTCCCTAAGGGTTGGATGTGGGACTAGCATTGCCACCTCCTTTGCCCTAAGAGAAACCTCCCAGAACATCTCACAGCATTTCCATCTTTTGTCCTTTGCAG | |
| M‐GLDC‐E25D | *CCCTTCGTGAAACCAGAGAACAAATTCTGGCCAACGATTGCCCGGATTGATGACATATCTAGATTGGATCTTGCTGGCAC | |
| GLDCP | M‐GLDCP‐1D | *AGCATTGATGAATTGATCGAGAAGTCTAGATTGGATCTTGCTGGCAC |
| EXT2 | M‐EXT2‐E13U | GGGTTCCCTAAGGGTTGGACAGCCATAGATGGGCTTTCACT |
| M‐EXT2‐E13D | *AGACCAAACACACATGGTGGATCTAGATTGGATCTTGCTGGCAC | |
| AMT | M‐AMT‐E1U | GGGTTCCCTAAGGGTTGGAGATGCAGAGGGCTGTAAGTGTGGTG |
| M‐AMT‐E1D | *GCCCGTCTGGGCTTTCGCCTGTCTAGATTGGATCTTGCTGGCAC | |
| M‐AMT‐E4U | GGGTTCCCTAAGGGTTGGAAGGGCCACCTGTATGTGGTGTCCAAC | |
| M‐AMT‐E4D | *GCTGGCTGCTGGGAGAAAGATTTTCTAGATTGGATCTTGCTGGCAC | |
| M‐AMT‐E9U‐2 | GGGTTCCCTAAGGGTTGGATGATGCGTGGCTTATGCTTGCTTGACAG | |
| M‐AMT‐E9D‐2 | *GTACTGTGACTAGTGGCTGCCCCTTCTAGATTGGATCTTGCTGGCAC |
Single‐underlined and double‐underlined sequences are binding sites for forward and reverse polymerase chain reaction (PCR) primers, respectively.
*5′ phosphorylation.