Figure 7.

SnoN localizes to PML nuclear subdomains. A, Transient overexpression of wild type SnoN‐EGFP was performed by nucleofection and Western analysis of SnoN was performed with a polyclonal SnoN antibody which detected SnoN‐EGFP fusion protein. GAPDH was used as a loading control. B, Transient overexpression of wild type SnoN expressed as an EGFP fusion protein was performed by nucleofection and immunofluorescence staining was performed to examine localization of EGFP and counterstained with propidium iodide (PI). C, TIOSE cells expressing SnoN‐EGFP and PML isoforms (PML1, PML2, PML3, PML4, or PML5) by nucleofection and plated onto glass coverslips. Cells were then stained with PML antibodies and viewed under an inverted fluorescence microscope to detect colocalization of SnoN‐EGFP with PML.