Figure 8.

Knock‐down of PML decreases SnoN‐induced increases in p21 levels. A, B, Both control and SnoN‐EGFP F‐11 cells were transfected with PML siRNA. Following 24 and 48h transfection with PML siRNA, RNA and protein were harvested, respectively. qPCR was performed using PML qPCR probes/primers to measure RNA‐fold changes in PML RNA levels and Western blotting was performed using antibodies against PML, SnoN, p21, and GAPDH as a loading control. C, Control and PML siRNA treated TIOSE cells at 24h post‐transfection, were re‐seeded on to 96 well plates at 5000 cells were well and allowed to grow for another 48h. Growth rate was measured by MTS assay.