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. 2008 Dec 9;105(51):20055–20062. doi: 10.1073/pnas.0810241105

Fig. 3.

Fig. 3.

Specificity of miR173 for syn-tasiRNA formation in N. benthamiana transient assays. (A) Flow chart. (B) Syn-tasiRNA constructs containing miR173 or miR171 target sites were expressed or coexpressed as indicated above blot panels. One of two biological replicates is shown. Probe sequences for 5′ and 3′ regions of syn-tasiRNA constructs are indicated by horizontal arrows in Fig. 2A. EtBr-stained 5S rRNA/tRNA are shown as a loading control. (C) Blot assays for syn-tasiRNA full-length transcripts and cleavage products (cle. prod.). The ratios of 3′ cleavage product to full-length transcript are shown above the blot images. EtBr-stained 18S/25S rRNA are shown as a loading control. (D) EtBr-stained gel of 5′ RACE products. 5′ RACE products corresponding to cleavage at the canonical miRNA-guided cleavage site migrate at 373 bp. ACTIN RT-PCR products are shown as a control.