Fig. 1.
Characterization of SMRTmRID mice. (A) Schematic of SMRT functional domains. (B) Mammalian 2-hybrid experiment showing interaction of wt and SMRTmRID Gal4-SMRT and indicated VP16-NHR-LBD fusion proteins in CV-1 cells. (C) Reduced body weights in SMRTmRID mice compared with wt littermates are observed from 8 to 24 weeks of age. (D) H&E-stained sections of SMRTmRID brains in P0 and adult mice reveal no apparent defects. Corpus callosum (cc) and anterior commissure (aca, acp) are indicated. (E) H&E-stained sections of SMRTmRID heart reveal a thinner ventricular wall (boxed area shown magnified in lower left corner) at P0 compared with wt. By 4 months of age, SMRTmRID hearts exhibit normal ventricular wall thickness.