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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Gastroenterology. 2008 Apr 22;135(2):529ā€“538. doi: 10.1053/j.gastro.2008.04.020

Figure 1. Expression of the CD4-TLR4 transgene in V-TLR4 mice.

Figure 1

A) Diagram of the V-TLR4 transgene. The transgene encodes a fusion protein containing the mouse CD4 (mCD4) extracellular domain and human TLR4 signaling domain and is driven by the mouse villin promoter (mVillin). p(A) represents SV40 poly A sequences. B) CD4-TLR4 mRNA expression in different tissues of V-TLR4 mice. The values were standardized to ubiquitin levels in each sample. H: Heart; Lu: Lung; K: kidney; Li: liver; P: Pancreas; M: Skeletal Muscle; Sk: Skin; T: Testis; Br: Brain; LI: Large intestine; SI: Small intestine. Cā€“D). Representative immunostaining for CD4 (red) and DAPI (blue) in small intestine of WT (C) and V-TLR4 (D) mice. E) Representative immunostaining for CD3 (red) and CD4 (green) in small intestine of V-TLR4 mice. Note that the transgene expression is restricted to epithelial cells. F) FACS analysis of CD4-TLR4 expression in epithelial cells isolated from small intestine. Cells were gated on the CD45āˆ’/PIāˆ’.