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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Gastroenterology. 2008 Apr 22;135(2):529–538. doi: 10.1053/j.gastro.2008.04.020

Figure 6. Increase in APRIL expression in IECs and B cell class switching to IgA in V-TLR4 mice.

Figure 6

A). Q-PCR analysis of APRIL, BAFF and TSLP expression in proximal small intestinal epithelial cells of WT and V-TLR4 mice (n=3/group; **, p<0.01). B). Western blot of APRIL protein in proximal small intestinal epithelial cells of WT and V-TLR4 mice. C & D). Immunostaining of APRIL in duodenum of WT (C) and V-TLR4 (D) mice (red, APRIL; blue, DAPI). E). FACS analysis of IgM+/IgA+ cells in lamina propria of proximal small intestine of WT and V-TLR4 mice (WT n=3, V-TLR4 n=4; * p< 0.05). F). RT-PCR analysis of post switch circular transcript (α-CT) expression in lamina propria B cells from WT and V-TLR4 mice. Data represented pooled B cells from 20 WT or 10 V-TLR4 mice. Different bands represent different splice variants of α-CT as previously described 24.