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. Author manuscript; available in PMC: 2008 Dec 16.

Published in final edited form as: Biochemistry. 2008 Dec 16;47(50):13383–13393. doi: 10.1021/bi801492x

Table 4.

FRET distances from actin C374 to cTnI in cardiac thin filaments reconstituted with bisphosphorylated cTnI

		Thin Filament		Thin Filament + S1-ADP
Distance	Conditions	Mean Distance (Å)	Half-Width (Å)	Mean Distance (Å)	Half-Width (Å)
p-cTnI(131C)-actin(C374)	Mg²⁺	44.3 (43.1, 45.6)	11.5 (10.2, 13.6)	46.4 (44.3, 58.3)	12.4 (11.9, 15.9)
p-cTnI(131C)-actin(C374)	Mg²⁺ + Ca²⁺	44.3 (43.4, 45.4)	12.0 (10.8, 13.3)	49.5 (48.4, 50.3)	14.4 (12.7, 16.9)
p-cTnI(151C)-actin(C374)	Mg²⁺	44.9 (43.6, 55.7)	9.5 (6.9, 11.6)	53.5 (52.1, 54.5)	11.7 (11.0, 12.4))
p-cTnI(151C)-actin(C374)	Mg²⁺ + Ca²⁺	53.4 (52.0, 55.2)	7.0 (6.2, 7.7)	59.0 (48.0, 50.3)	10.1 (9.3, 11.1)
p-cTnI(160C)-actin(C374)	Mg²⁺	48.3 (47.1, 49.6)	10.3 (9.4, 11.1)	56.2 (55.1, 57.7)	9.9 (8.3, 11.4))
p-cTnI(160C)-actin(C374)	Mg²⁺ + Ca²⁺	58.0 (56.8, 59.3)	11.1 (9.7, 12.8)	60.1 (58.9, 61.6)	12.8 (11.1, 14.3))
p-cTnI(167C)-actin(C374)	Mg²⁺	42.1 (41.0, 43.4)	15.1 (14.2, 15.9)	55.4 (54.2, 56.8)	16.2 (15.6, 16.9)
p-cTnI(167C)-actin(C374)	Mg²⁺ + Ca²⁺	54.0 (52.7, 55.4)	12.7 (10.9, 14.3)	60.0 (59.0, 61.4)	11.7 (11.1, 12.7)

• Numbers in parentheses are the lower and upper bounds of the best fitted parameters at the 68% confidence level (one standard deviation.