FIGURE 8.

Test results for a hop diffusion data set including effects of diffusion during illumination. We simulated trajectories with exponentially distributed length (mean 10 observations) and selected a subset of 500 trajectories with more than 5 observations for analysis. The set point for X was at the parameter settings L₀ = 100 nm, D_macro = 0.3 μm²/s, and σ_xy,0 = 20 nm. Probe data sets Y were simulated in a parameter range L = 50–150 nm, and σ_xy = 0–40 nm, with a size of 10,000 trajectories. The plots show the decadic logarithm of the corrected p-values as a function of D and σ_xy. Display settings are identical to those of Fig. 4. A shows results for t_del = 2 ms and t_ill = 2 ms including data for n = 1, 2, and 3. For this setting, the set point can be well extracted. When combining data sets simulated at (t_ill = 0 ms, t_del = 4 ms), (t_ill = 2 ms, t_del = 2 ms), and (t_ill = 4 ms, t_del = 0 ms), the significance region can be further reduced (B).