Skip to main content
. 2008 Sep 26;95(12):5978–5987. doi: 10.1529/biophysj.108.136168

FIGURE 3.

FIGURE 3

(A) Raman spectra collected from the cytoplasm (spectrum 1) and nucleus (spectrum 2) of an early-culture ATII cell. The spectrum of phosphatidyl choline (PCh) is included for comparison with spectrum 1. The spectra have been corrected for instrument response, baseline-corrected, the wavenumber axis standardized and aligned, and the PCh spectrum scaled to represent equal maximum intensity to spectrum 1. (B) Light microscope picture of an early-culture ATII cell. (C) Same cell as in B, with sites of spectral acquisition labeled 1 and 2. Ellipses indicate the region probed by each scan. The spectra are offset for clarity. The correlation coefficient between spectrum 1 and the PCh spectrum is R = 0.97. Scale bar, 5 μm.