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. 2008 Sep 19;95(12):5890–5900. doi: 10.1529/biophysj.108.138081

FIGURE 3.

FIGURE 3

Comparison of the EPR spectra of the CoII-substrate radical pair state before annealing and at different levels of decay, for decay performed at T = 197 K. (A) CoII-substrate radical pair state generated by using 1,1,2,2-2H4-aminoethanol as substrate. (a) Preannealling spectrum, obtained at 160 K. (b) Average of spectra corresponding to amplitudes of 99–90% of initial amplitude. (c) Average of spectra corresponding to amplitudes of 79–70% of initial amplitude. (d) Average of spectra corresponding to amplitudes of 59–50% of initial amplitude. (B) CoII-substrate radical pair state generated by using 1-13C-aminoethanol as substrate. (a) Preannealling spectrum, obtained at 160 K. (b) Average of spectra corresponding to amplitudes of 99–90% of initial amplitude. (c) Average of spectra corresponding to amplitudes of 79–70% of initial amplitude. (d) Average of spectra corresponding to amplitudes of 59–50% of initial amplitude. Experimental conditions: (A) Microwave frequency, 9.3390 GHz; microwave power, 20.25 mW; magnetic field modulation, 1.0 mT; modulation frequency, 100 kHz; scan rate, 6.52 mT/s; and time constant, 2.56 ms. (B) Microwave frequency, 9.3390 GHz; microwave power, 20.25 mW; magnetic field modulation, 1.0 mT; modulation frequency, 100 kHz; scan rate, 6.52 mT/s; and time constant, 2.56 ms.