Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1987 Nov;55(11):2838–2840. doi: 10.1128/iai.55.11.2838-2840.1987

Protein variability among strains of Mycoplasma pulmonis.

H L Watson 1, M K Davidson 1, N R Cox 1, J K Davis 1, K Dybvig 1, G H Cassell 1
PMCID: PMC259986  PMID: 3312017

Abstract

The proteins of Mycoplasma pulmonis were examined by two-dimensional polyacrylamide gel electrophoresis and immunoblotting. Comparison of profiles from silver-stained two-dimensional polyacrylamide gel electrophoresis of 18 strains of M. pulmonis allowed identification of five proteins that were strain variable. These variable proteins were not dependent on the medium used to grow the organisms and were shown by reaction with serum samples from naturally infected rats and mice to be produced in vivo and not to be medium components. Identification of strain-variable proteins may lead to an explanation of the differences in properties found among M. pulmonis strains.

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cassell G. H. Derrick Edward Award Lecture. The pathogenic potential of mycoplasmas: Mycoplasma pulmonis as a model. Rev Infect Dis. 1982 May-Jun;4 (Suppl):S18–S34. doi: 10.1093/clinids/4.supplement_1.s18. [DOI] [PubMed] [Google Scholar]
  2. Cassell G. H., Lindsey J. R., Davis J. K., Davidson M. K., Brown M. B., Mayo J. G. Detection of natural Mycoplasma pulmonis infection in rats and mice by an enzyme linked immunosorbent assay (ELISA). Lab Anim Sci. 1981 Dec;31(6):676–682. [PubMed] [Google Scholar]
  3. Davidson M. K., Lindsey J. R., Brown M. B., Schoeb T. R., Cassell G. H. Comparison of methods for detection of Mycoplasma pulmonis in experimentally and naturally infected rats. J Clin Microbiol. 1981 Dec;14(6):646–655. doi: 10.1128/jcm.14.6.646-655.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Forshaw K. A., Fallon R. J. Serological heterogeneity of Mycoplasma pulmonis. J Gen Microbiol. 1972 Oct;72(3):501–510. doi: 10.1099/00221287-72-3-501. [DOI] [PubMed] [Google Scholar]
  5. Horowitz S. A., Garrett B., Davis J. K., Cassell G. H. Isolation of Mycoplasma pulmonis membranes and identification of surface antigens. Infect Immun. 1987 May;55(5):1314–1320. doi: 10.1128/iai.55.5.1314-1320.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Howard C. J., Taylor G. Variation in the virulence of strains of Mycoplasma pulmonis related to susceptibility to killing by macrophages in vivo. J Gen Microbiol. 1979 Oct;114(2):289–294. doi: 10.1099/00221287-114-2-289. [DOI] [PubMed] [Google Scholar]
  7. Merril C. R., Dunau M. L., Goldman D. A rapid sensitive silver stain for polypeptides in polyacrylamide gels. Anal Biochem. 1981 Jan 1;110(1):201–207. doi: 10.1016/0003-2697(81)90136-6. [DOI] [PubMed] [Google Scholar]
  8. Minion F. C., Brown M. B., Cassell G. H. Identification of cross-reactive antigens between Mycoplasma pulmonis and Mycoplasma arthritidis. Infect Immun. 1984 Jan;43(1):115–121. doi: 10.1128/iai.43.1.115-121.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Naot Y., Tully J. G., Ginsburg H. Lymphocyte activation by various Mycoplasma strains and species. Infect Immun. 1977 Nov;18(2):310–317. doi: 10.1128/iai.18.2.310-317.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nichols E. J., Kenny G. E. Immunochemical characterization of a heat-stable surface antigen of Mycoplasma pulmonis expressing both species-specific and strain-specific determinants. Infect Immun. 1984 May;44(2):355–363. doi: 10.1128/iai.44.2.355-363.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  12. Razin S. Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985 Dec;49(4):419–455. doi: 10.1128/mr.49.4.419-455.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Taylor-Robinson D., Furr P. M., Davies H. A., Manchee R. J., Mouches C., Bove J. M. Mycoplasmal adherence with particular reference to the pathogenicity of Mycoplasma pulmonis. Isr J Med Sci. 1981 Jul;17(7):599–603. [PubMed] [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES