Figure 10.
Influenza A, but not RSV, causes a cytopathic effect in polarized 16HBE14o- human bronchial epithelial cells. Cells grown in Transwells were infected apically with RSV or influenza A virus and incubated for 1 hour Infection medium was removed, cells were further incubated for 23 hours and RT of cell cultures (A) and LDH levels in basolateral medium (B) were measured. In some experiments, cells were infected with RSV (C) or influenza A virus (D) as described above, fixed in methanol, and immunostained with antibody to ZO-1. Results represent the mean of three independent experiments performed in triplicate; bars represent mean ± SEM (P < 0.05, analysis of variance). Arrows in panel D show loss of both ZO-1 and nuclei.