Figure 11.

Rhinovirus (RV)1B disrupts airway epithelial tight junctions in vivo. C57BL/6 mice were infected with RV1B (5 × 10⁷ TCID₅₀) or an equal volume of sham. Mice were killed at 24 hours post-infection, and paraffin sections prepared from the lungs were deparaffinized, rehydrated, and immunostained with antibody to ZO-1. The bound antibody was detected by anti-rabbit IgG conjugated with AlexaFluor 488. (A and C) Large and small airways, respectively, from sham-infected mice. (B and D) Large and small airways, respectively, from RV1B-infected mice. Arrows represent absence of dissociation of zona occludins-1 from the tight junction complex.