Figure 3.
RV decreases the RT of polarized 16HBE14o- cells in a dose- and time-dependent manner. Cells grown in transwells were infected apically with (A) RV39 or (B) RV1B at doses ranging from 0.25 to 5 MOI and incubated for 1 hour. Infection medium was removed, incubation continued for another 23 hours and RT was measured. Cytotoxic effect was determined by measuring LDH levels in basolateral media (C). The time course of RV-induced reductions in RT was determined by infecting polarized cell monolayers with RV39 at MOI of 1 for 1 hour, replacing the infection media with fresh cell culture media, and measuring RT measured at the indicated times (D). Data represent the mean and standard error of four independent experiments carried out in triplicate (P < 0.05, analysis of variance).