Figure 5.
Apoptosis does not contribute to rhinovirus (RV)-induced reductions in transepithelial resistance (RT). (A) Primary diffentiated airway epithelial cells or (B) polarized 16HBE14o- human bronchial epithelial cells were infected with RV39 or sham or treated with 5 μM thepsigargin for 1 hour. Media-treated cells were used as negative controls. Cells were stained with mixture of FITC-annexin V and propidium iodide and analyzed by flow cytometry. Results represent the mean of three independent experiments performed in triplicate; bars represent mean ± SEM (P < 0.05, analysis of variance).