Figure 1.

TRPC6 per se is not mechanosensitive. (A) Whole-cell recordings from HEK293 cells expressing TRPC6; application of hypotonic stimulus (250 mOsm kg⁻¹), ‘Hypo', and of 100 μM OAG are indicated. Current time courses at ±60 mV, zero current levels (stippled lines), time scale bar (50 s) and current scale bar (200 pA). (B–E) Single-channel recordings in inside-out patches with negative pipette pressure and subsequent SAG bath application. (B) Original current trace, scale bar (2 pA, 10 s) (top); 13 consecutive traces on an expanded time-scale at the indicated time points, scale bar (5 pA, 20 ms) (middle); the respective graph of consecutive open probabilities (NPo) in 1-s steps (bottom) is displayed. (C, D) Analysis of mean NPo values. (E) Representative expanded current traces around the time point of pressure application, scale bar (5 pA, 10 ms).