Figure 4.

Osmotically induced membrane stretch activates phospholipase C and G proteins. (A–D) Whole-cell recordings from HEK293 cells co-expressing TRPC6 and H₁R. Recording with 10 μM U73122 (A) or with 10 μM U73343 (B) in the pipette solution. Recordings of cells pretreated with 100 ng ml⁻¹ PTX for 16 h (C) or with 2 mM GDP-β-S in the pipette solution (D). IV relationships before ‘Basal', during hypotonic stimulation, ‘Hypo' and receptor stimulation with 100 μM histamine, ‘His' are shown. (A–D) Insets show current time courses at ±60 mV, hypotonic and histamine stimulations, zero current levels (stippled lines); scale bars: 500 pA, 50 s.