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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Dev Biol. 2008 Jun 21;321(1):227–237. doi: 10.1016/j.ydbio.2008.06.020

FIGURE 2. The SUMO E2 conjugating enzyme, UBE2I, localizes to synaptonemal complexes, the XY body and centromeres in spermatocytes.

FIGURE 2

Surface-spread meiotic chromatin was immunolabeled with a mouse monoclonal antibody specific for the synaptonemal complex marker SYCP3 (red) and a rabbit polyclonal antibody specific for the SUMO E2 conjugating enzyme UBE2I (green). A) UBE2I localizes to the XY body (arrow) and associates with the synaptonemal complex in pachytene spermatocytes (open arrow). B) Visualization of chromatin using the fluorescent dye DAPI - same cell as in A. C) UBE2I also localizes to the XY body (arrow) and the synaptonemal complex (open arrow) in diplotene spermatocytes. D) Visualization of chromatin using the fluorescent dye DAPI - same cell as in C. E) Exclusive localization of UBE2I to centromeres (open arrow) as seen by the co-localization of the UBE2I signal with that of SYCP3 to give a yellowish signal in MI spermatocytes. F) Visualization of chromatin using the fluorescent dye DAPI - same cell as in E. Magnification = 1000X.