Table 4. Summary of cleavage scores predicted for cleavage junctions of proteins of West Nile virus strains*.

	SA381/ 00	H442	SPU116/ 89	SA93/01	B956D117B3	AnMg798	NY-385–99	NY-385–99 clone TYP-9376	NY-385–99 clone 9317B	TM171–03	MRM61C	p value†
Between capsid and premembrane proteins
G	+3.69	+3.69	+3.69	+3.69	+3.69	+4.00	−0.49	−0.49	−0.49	−0.49	−1.85	0.00005
A‡	+9.37	+9.37	+9.37	+9.37	+9.37	+8.01	+5.93	+5.93	+5.93	+5.93	+7.37	0.00004
V	−9.14	−9.14	−9.14	−9.14	−9.14	−7.8	−9.52	−9.52	−9.52	−9.52	−10.32	0.02235
Between premembrane and envelope proteins
Y	−10.15	−10.15	−10.15	−10.15	−10.15	−9.12	−9.12	−9.12	−9.12	−9.12	−9.45	0.00433
S‡	+11.27	+11.27	+11.27	+11.27	+11.27	+12.42	+12.42	+12.42	+12.42	+12.42	+11.50	0.01728
F	−5.37	−5.37	−5.37	−5.37	−5.37	−4.78	−4.78	−4.78	−4.78	−4.78	−5.27	0.01977
Between envelope protein and nonstructural protein 1
H	−9.01	−9.01	−9.01	−9.01	−9.01	−9.71	−9.01	−9.01	−9.01	−9.01	−9.01	0.36322
A‡	+4.26	+4.26	+4.26	+4.26	+4.26	+4.04	+4.26	+4.26	+4.26	+4.26	+4.26	0.36322
D	−11.05	−11.05	−11.05	−11.05	−11.05	−11.15	−11.05	−11.05	−11.05	−11.05	−11.05	0.36322
Between nonstructural proteins 4B and 5
R	−16.05	−16.05	−16.05	−16.05	−16.05	−16.05	−16.05	−16.05	−16.05	−15.83	−16.05	0.37390
G‡	−13.19	−13.19	−13.19	−13.19	−13.19	−13.19	−13.19	−13.19	−13.19	−13.08	−13.19	0.37390
G	−19.54	−19.54	−19.54	−19.54	−19.54	−19.54	−19.54	−19.54	−19.54	−19.66	−19.54	0.37390

*Signal cleavage predicted scores were calculated with AnalyzeSignalase 2.03 (14). The table indicates only the last amino acid of the first protein and the first 2 amino acids of the following protein.
†Two-tailed Student t test results, indicating the probability of significance of observed differences between lineage 2 and lineage 1 strains.
‡Exact cleavage site.