Table. Primers used for the PCR investigation of the clinical sample from Singapore*.

Primers	Forward	Sequence (5′ → 3’)	Reverse	Sequence (5′ → 3’)	Results
Nest 1, genus specific primers
Genus specific (11)	rPLU 1	TCA AAG AAT AAG CCA TGC AAG TGA	rPLU 2	TAC CCT GTT GTT GCC TTA AAC TCC	+
Nest 2, genus- and species-specific primers
Genus specific (11)	rPLU 3	TTT TTA TAA GGA TAA CTA CGG AAA AGC TGT	rPLU 4	TAC CCG TCA TAG CCA TGT TAG GCC AAT ACC	+
Plasmodium knowlesi specific (5)	Pmk8	GTT AGC GAG AGC CAC AAA AAA GCG AAT	Pmkr9	ACT CAA AGT AAC AAA ATC TTC CGT A	+
P. vivax specific (11)	rVIV1	CGC TTC TAG CTT AAT CCA CAT AAC TGA TAC	rVIV2	ACT TCC AAG CCG AAG CAA AGA AAG TCC TTA	–
P. falciparum specific (11)	rFAL1	TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT	rFAL2	ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC	–
P. malariae specific (11)	rMAL1	ATA ACA TAG TTG TAC GTT AAG AAT AAC CGC	rMAL2	AAA ATT CCC ATG CAT AAA AAA TTA TAC AAA	–
P. ovale specific (11)	rOVA1	ATC TCT TTT GCT ATT TTT TAG TAT TGG AGA	rOVA2	GGA AAA GGA CAC ATT AAT TGT ATC CTA GTG	–

*PCR was carried out at the Environmental Health Institute and cycling conditions used were as described in the references shown in parentheses. 
+, positive; –,negative.