Table 2. Primers used to detect tet genes and conjugative transposon Tn916 in Streptococcus suis*.

Gene	Primers (5′ → 3′)	Product size, bp	Annealing temperature, °C
tetA	GCTACATCCTGCTTGCCTTC; CATAGATCGCCGTGAAGAGG	210	55
tetB	TTGGTTAGGGGCAAGTTTTG; GTAATGGGCCAATAACACCG	659	55
tetC	CTTGAGAGCCTTCAACCCAG; ATGGTCGTCATCTACCTGCC	418	55
tetD	AAACCATTACGGCATTCTGC; GACCGGATACACCATCCATC	787	55
tetE	AAACCACATCCTCCATACGC; AAATAGGCCACAACCGTCAG	278	55
tetG	GCTCGGTGGTATCTCTGCTC; AGCAACAGAATCGGGAACAC	468	55
tetK	TCGATAGGAACAGCAGTA; CAGCAGATCCTACTCCTT	169	55
tetL	TCGTTAGCGTGCTGTCATTC; GTATCCCACCAATGTAGCCG	267	55
tetM	GTGGACAAAGGTACAACGAG; CGGTAAAGTTCGTCACACAC	406	55
tetO	AACTTAGGCATTCTGGCTCAC; TCCCACTGTTCCATATCGTCA	515	55
tetQ	TTATACTTCCTCCGGCATCG; ATCGGTTCGAGAATGTCCAC	904	55
tetS	CATAGACAAGCCGTTGACC; ATGTTTTTGGAACGCCAGAG	667	55
ORF 24	ATGAGGTGTCTATTTTTTTA; TTATTGGCTGAATGAATGTT	120	52
ORF 23	AATTTGTGATTCCCAACATG; CGTCAGCATGTAAAAGGTAA	315	52
ORF 22	ATGATGAGATTAGCAAATGG; CTATTTGTCTTGTGTCGGTT	387	52
ORF 21	TTTCATTTTACGATAGCGTC; GTCGCCTGCGTGGACTGTCT	1,308	55
ORF 20	ATGCTGTTTGATTATGTAAG; TTATTTTTTTGTTGTTATCA	990	52
ORF 19	ATGAATTTTGGACAAAACCT; TTAAGCACCAATAATGCGAT	222	52
ORF 18	TTTAGGCAAATACAATGAGG; GATTGGTTGAGATAAACGTT	443	56
ORF 17	ATGGGATTTTTGAAATCGTC; TTAATTGGATATGCCATAAA	507	52
ORF 16	ATGGCATATCCAATTAAATA; TTACACCTCTTTTCGCACAG	2,448	52
ORF 15	ATGTGAAACCATCAATAGTA; TCATCTGAAAATAAAATGGC	2,265	52
ORF 14	ATGAAGTTGAAAACTTTAGT; TCATTGTTTGATTCGTCCTG	1,002	52
ORF 13	AGAAAAACAGATACCAAAGG; CGTTCTTTTCAAGTACCAAA	860	54
ORF 12-tetM	ATGCTTTGTATGCCTATGGT; CTAAGTTATTTTATTGAACA	2022	54
ORF 5–10	ATTATAAACTACAAGTGGAT; TTCGTTTAATCTGAATACGA	2233	52
Xis-Tn	ATGAAGCAGACTGACATTCC; TTCGTTTAATCTGAATACGA	204	52
Int-Tn	GACTGGAGAGAGCCAACGAA; CATCATGCCGTTGTAATCAC	925	54

*tet, tetracycline; ORF, open reading frame.