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. 2008 Jul;14(7):1132–1134. doi: 10.3201/eid1407.071468

Table 2. Serologic testing results for highly pathogenic avian influenza virus (H5N1) in calves, Germany, 2007*.

Calf Day postinoculation
0
7
14
21
28
91
NP† VN‡ HI§ NP VN HI NP VN HI NP VN HI NP VN HI NP VN HI
Inoculated
A1 98 2.3 <3 90 3.3 <3 42 4 <3 26 4 <3 24 6.2 3 20 7 <3
A2 114 0.4 <3 117 3 <3 69 5 <3 27 4 <3 22 6.2 3 18 6.7 3
A3 118 <1 <3 90 2.7 <3 28 5.7 <3 29 5 <3 21 7 3 15 7 3
A4
102
0.7
<3

122
2
<3

25
5.3
<3

24
5
<3

24
5.8
3

19
6.3
<3
Contact
K1 96 1.3 <3 88 <1 <3 73 2 <3 44 4 <3 40 4.5 <3 45 5 <3
K2 120 0.7 <3 98 2.3 <3 89 <1 <3 68 <1 <3 76 0.7 <3 50 <1 <3

*Inoculated calves received highly pathogenic influenza virus (H5N1) strain A/cat/Germany/R606/2006 (8,9); contact calves were not inoculated but were housed with the inoculated calves. Positive results are in boldface.
†NP, avian influenza A– blocking ELISA against nucleoprotein (Pourquier, Montpellier, France) inhibition % (<35, positive; >45, negative; 35–45, questionable).
‡VN, virus neutralization test (ND100 log2); values >4 are considered positive.
§HI, hemagglutination inhibition (log2); values >3 are considered positive.