Table 3. Description and performance characteristics of Zika virus real-time RT-PCR primer/probe sets*.
| Primer | Genome position† | Sequence (5′ → 3′) | Sensitivity, no. copies | Specificity‡ |
|---|---|---|---|---|
| ZIKV 835 | 835–857 | TTGGTCATGATACTGCTGATTGC | ||
| ZIKV 911c | 911–890 | CCTTCCACAAAGTCCCTATTGC | 100 | ZIKV |
| ZIKV 860-FAM | 860–886 | CGGCATACAGCATCAGGTGCATAGGAG | ||
| ZIKV 1086 | 1086–1102 | CCGCTGCCCAACACAAG | ||
| ZIKV 1162c | 1162–1139 | CCACTAACGTTCTTTTGCAGACAT | 25 | ZIKV |
| ZIKV 1107-FAM | 1107–1137 | AGCCTACCTTGACAAGCAGTCAGACACTCAA |
*RT-PCR, reverse transcription–PCR; ZIKV, Zika virus. †Based on ZIKV MR 766 GenBank accession no. AY632535. ‡ZIKV specificity indicates a positive result with ZIKV only and no reactivity with dengue virus-1 (DENV-1), DENV-2, DENV-3, DENV-4, West Nile virus, St. Louis encephalitis virus, yellow fever virus, Powassan virus, Semliki Forest virus, o’nyong-nyong virus, chikungunya virus, and Spondweni virus.