Figure 2. Synthetic dsRNA and dsDNA cause MAVS cleavage and apoptosis.
HeLa cells were incubated (A, C), or transfected using Lipofectamine (Lipo; A–C), with Poly(I:C) or Poly(dAT:dAT). In (A), 10 µg ml−1 Poly(I:C) was added to the culture 15 medium or transfected into cells for h. In (B), Poly(I:C) was transfected into cells for 6 h at the indicated concentrations per ml. In (C), Poly(I:C) or Poly(dAT:dAT) were added to the culture medium or transfected into cells for 6 h at 10 µg ml−1. Transfections occurred in the presence or absence of 10 µM MG132 as noted. After 6 h, cells extracts were analyzed by Western blot.