Table 2.

Identification of Vitreous Human Proteins. Spot numbers correspond to those shown in Figures 4 and 5.

Spot #	Protein Identify	Gene Symbol	New or Previously Reported (+)	Theoretical MW / pl	Accession No.	Score a)	Sequence Coverage (%)	Peptide Matched (%)b)
1	complement factor B	BF	New	87155 / 6.6	2347133	90	12	73
2	gelsolin isoform b	GSN	New	80946 / 5.6	38044288	93	26	50
3	complement component 3	C3	+	188948 / 6.0	40786791	136	19	68
4	fibrinogen γ	FGG	+	46963 / 5.5	223170	114	20	90
5	alpha1-B-glycoprotein	A1BG	+	52619 / 5.7	69990	117	25	63
6	serum albumin	ALB	+	71835 / 6.1	23307793	139	22	68
7	alpha1-antitrypsin	AAT*	+	44294 / 5.4	1942629	126	39	57
8	alpha1-antitrypsin	AAT*	+	44294 / 5.4	1942629	107	29	43
9	alpha2-HS glycoprotein	AHSG	+	40393 / 5.4	2521983	60	13	50
10	alpha2-HS glycoprotein	AHSG	+	40393 / 5.4	2521983	91	18	62
11	fibrinogen γ	FGG	+	46963 / 5.5	223170	121	41	50
12	fibrinogen β chain preproprotein C)	FGB	New	56745 / 8.5	70906435	110	38	50
13	serum albumin	ALB	+	48964 / 6.0	27692693	98	19	69
14	zinc alpha-2-glycoprotein 1	AZGP1	+	34507 / 5.7	52790422	154	43	61
15	Apolipoprotein A-IV	APOA4	+	45371 / 5.3	37499461	188	50	63
16	proapolipoprotein, proapolipoprotein A-I	APOA1	+	28944 / 5.5	178775	234	56	78
17	alpha1-antitrypsin	AAT*	New	44294 / 5.4	1942629	116	28	61
18	transferrin	TF	+	79841 / 6.8	31415705	317	46	85
19	complement component 3	C3	+	188948 / 6.0	40786791	105	9	75
20	antithrombin III	SERPINC1	+	53137 / 6.3	179161	90	32	52
21	vitamin D-binding protein, group specific component	GC	+	54905 / 5.3	455970	78	16	78
22	vitamin D-binding protein, group specific component	GC	+	54905 / 5.3	455970	134	29	63
23	vitamin D-binding protein, group specific component	GC	+	54905 / 5.3	455970	108	24	50
24	complement component 3	C3	+	188948 / 6.0	40786791	83	10	53
25	clusterin, SP40/40, apoliporotein	CLU	+	37067 / 5.7	338305	87	30	47
26	clusterin, SP40/40, apoliporotein	CLU	+	37067 / 5.7	338305	89	25	100
27	clusterin, SP40/40, apoliporotein	CLU	+	37067 / 5.7	338305	67	23	63
28	transthyretin	TTR	+	12836 / 5.3	339685	133	91	57
29	complement component 4A	C4A	New	30380 / 6.3	57209751	96	28	70
30	prostaglandin D2 synthase	PTGDS	+	23106 / 9.9	55962673	130	41	71
31	amyloid P component, serum	APCS	New	25513 / 6.6	55665341	75	24	56
32	prostaglandin D2 synthase	PTGDS	+	23106 / 9.9	55962673	53	24	50
33	proapolipoprotein, proapolipoprotein A-I	APOA1	+	28944 / 5.5	178775	128	53	54
34	serum albumin	ALB	+	19900 / 5.3	37222202	146	53	41
35	plasma glutathione peroxidase	GPX3	+	16788 / 8.9	404108	115	57	69
36	plasma retinal binding protein 4	RBP4	+	23355 / 5.8	55663330	101	47	67
37	not determined d)		Unknown
38	transthyretin	TTR	+	12836 / 5.3	339685	113	62	54
39	transthyretin	TTR	+	12836 / 5.3	339685	146	71	56
40	fibrin β	fibrin β*	New	51512 / 8.0	223002	173	51	69
41	cathepsin D	CTSD	+	45163 / 6.1	16740920	98	20	82
42	pigment epithelium-derived factor	PEDF*	+	46526 / 6.0	15217079	91	19	88
43	pigment epithelium-derived factor	PEDF*	+	46526 / 6.0	15217079	105	23	89
44	cathepsin D	CTSD	+	45163 / 6.1	16740920	78	20	53
45	pigment epithelium-derived factor	PEDF*	+	46526 / 6.0	15217079	104	34	45
46	pigment epithelium-derived factor	PEDF*	+	46526 / 6.0	15217079	103	20	67
47	cathepsin D	CTSD	+	45163 / 6.1	16740920	97	26	58
48	pigment epithelium-derived factor	PEDF*	+	46526 / 6.0	15217079	139	46	41

^a)Probability-based MOWSE score. Scores higher than 64 indicate the level of statistical significance at P<0.05.

^b)The percentage of matched peptides compared to the observed.

^c)The spot was identified as fibrinogen β after performing MS/MS followed by MASCOT search.

^d)The corresponding spot on plasma gel was identified as hp2-alpha. The peptide mass fingerprinting spectra highly resembled each other.

^*Not Gene Symbol.