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(A) In a SyrP-mediated reaction, formation of the L-threo-3-OH-[14C]Asp product is proportional to the amount of SyrE-A8T8-S-L-[14C]-Asp substrate. (B) When SyrE-A8T8-S-L-[14C]-Asp is held constant at 4 nmol and SyrP is titrated, multiple turnovers are observed at low SyrP:A8T8 ratios, indicating that SyrP functions catalytically.
