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. Author manuscript; available in PMC: 2008 Dec 10.
Published in final edited form as: Cancer Ther. 2008;6(B):865–876.

Figure 2.

Figure 2

RT-PCR analysis of PATZ1 gene knockdown in a glioma cells after transfection with siRNA. Cells were either not transfected (NT) or transfected with siRNA to either a randomized sequence (rand) or to PATZ1. Total RNA was isolated from the cells 48 hr post transfection and converted to cDNA. Real time PCR was performed using gene specific primers and SYBR green detection on an iQ5 ribocycler (BioRad). All data were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) prior to comparison for fold changes in mRNA levels determined from numerical readings taken from the ribocycler.