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. Author manuscript; available in PMC: 2009 Aug 8.
Published in final edited form as: Mol Cell. 2008 Aug 8;31(3):360–370. doi: 10.1016/j.molcel.2008.07.005

Figure 3. Effects of NMD mutations combined with inactivation of Rrp6p, Mlp1 or of Rnt1 stem-loops on the accumulation of unspliced pre-mRNAs.

Figure 3

A. Expression of RPGs and detection of unspliced RPGs in wild-type, upf1Δ and rrp6Δ and upf1Δrrp6Δ strains for NMD-sensitive transcripts. For some transcripts, different exposures are shown to properly visualize mRNAs. Legends as in Fig. 2. B. Expression of RPGs and detection of unspliced RPGs in wild-type, upf1Δ and mlp1Δ and upf1Δmlp1Δ strains for NMD-sensitive transcripts. Legends as in Fig. 2. C. Expression of RPGs and lack of detection of unspliced RPGs in wild-type, upf1Δ, mlp1Δ, rrp6Δ, upf1Δmlp1Δ and upf1Δrrp6Δ strains for NMD-insensitive transcripts. Legends as in Fig. 2. D. Inactivation of NMD and of Rrp6 shows synergistic effects on the accumulation of two unspliced pre-mRNAs. Legends as in A. E. Analysis of RPS22B in strains carrying a deletion of the Rnt1p-target stem-loops (SL1,2Δ) and/or carrying mutations of NMD components. Shown are the signals obtained by hybridization of the same membrane with the indicated probes.