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An example of a multiplex PCR protocol for the detection of prostate-specific antigen (PSA), prostate-specific membrane antigen (PSMA), prostate stem-cell antigen (PSCA), parathyroid hormone–related protein (PTHrP), and cytokeratin 19 (CK19) mRNA in a peripheral blood sample from a prostate cancer patient. Lane M, size markers; lane A, sample from prostate cancer patient with metastatic disease; lane B, sample from healthy young male (negative control).
