Table 5. Activity of several enzymes and the levels of proteins in the left lens of rats 13 weeks after injection.
| Time | Group I (n=5) | Group II (n=7) | Group III (n=5) | Group IV (n=7) | Group V (n=7) | Group VI (n=5) | Group VII (n=10) |
|---|---|---|---|---|---|---|---|
| CAT |
34.0±21.2 |
96.6±62.5a |
111.0±69.0b |
94.9±21.2 |
22.6±18.2 |
14.0±15.2 |
28.7±20.3* |
| GR |
5.2±2.7 |
7.6±3.9c,e |
10.4±3.1d,e |
5.6±5.0 |
5.5±3.6 |
4.1±3.7 |
5.5±4.3 |
| GSH |
102.7±39 |
76.3±19.5 |
41.4±13.8 |
42.5±22.1 |
66.3±26.9 |
75.7±45.2 |
55.2±32.6** |
| Lens water-soluble proteins | 4.9±0.4 | 4.4±0.5f | 4.32±0.9f | 4.4±0.7f | 4.3±0.5f | 4.4±0.5f | 4.7±0.4** |
The data are the mean±SD. CAT (catalase) is expressed as µmol/g lens protein. GSH (glutathione) is expressed as mg/g lens protein. GR (glutathione reductase) is expressed as µmol/g lens protein. The water-soluble protein is expressed as g/l. An asterisk indicates that p<0.05 (group I compared with group VII). A double asterisk indicates that p<0.001 (group I compared with group VII). Note: ap<0. 001 (group II compared with group VII); bp=0.002 (group III compared with group VII); cp=0.003 (group II compared with group VII); dp<0.001 (group III compared with group VII); ep<0.05 (groups II-III compared with group I); fp<0. 001 (group II-VI compared with group I). There were no significant differences in the lens water-soluble proteins in any of the six diabetic groups (Group II-VII). Group I: Normal; Group II: Carnosine treated (5 mg/ml); Group III: Carnosine treated (10 mg/ml); Group IV: Aspirin treated; Group V: Aminoguanidine treated (1 mg/ml); Group VI: Aminoguanidine treated (2 mg/ml); and Group VII: Untreated diabetic.