Table 1.
Relative percentage of VGLUT1 and VGAT mRNA levels compared to β-actin expression.
| Fluorescence-activated cell sorting | |||
| Sample | GFP Phenotype | VGLUT1 | VGAT |
| Immature cortical cells | Negative | 2.37% | n. d.* |
| Positive | n. d. | 7.89% | |
| Adult cortical cells | Negative | 3.91% | n. d. |
| Positive | n. d. | 9.51% | |
| Laser capture microdissection | |||
| Region | GFP Phenotype | VGLUT1 | VGAT |
| Reticular Nucleus | Positive | n. d. | 12.68% |
| Caudate | Positive | n. d. | 14.07% |
| Purkinje cells | Positive | n. d. | 15.39% |
| Hippocampal interneurons (CA1) | Positive | n. d. | 12.45% |
| Hippocampal pyramidal cells (CA1) | Negative | 11.95% | n. d. |
| Cerebellar granule cells | Negative | 13.56% | n. d. |
* n. d. (not detected) is considered less than 0.001% (i.e. Ct value > 10 cycles) of gene expression in comparable amounts of whole brain samples (see Materials and Methods).