Skip to main content
. Author manuscript; available in PMC: 2008 Dec 11.
Published in final edited form as: Cent Nerv Syst Agents Med Chem. 2008;8(3):203–219. doi: 10.2174/187152408785699613

Figure 4. Hydrogel constructs permit the co-culture of glia and endothelial cells, thereby enabling direct interactions between cell types.

Figure 4

A. LRM 55 astroglial cells (green) and bovine aortic endothelial cells (BAEC; red) were transfected using an Amaxa Nucleofector (Amaxa, Gaithersburg, MD). Cells were transfected as separate populations, with eGFP for LRM55 and mCherry for BAEC. A Leica SP5 confocal microscope (Leica, Bannockburn, IL) was used to image live samples after 7 days in culture. This type of culture system permits direct interactions between cells at the alginate hydrogel surface. B. Cell constructs were created above arrays of microfabricated electrodes, to enable TEER measurements. Each electrode array consisted of four 3x3 mm low-impedance Au electrodes. An Ag/AgCl reference electrode and a Pt foil counter electrode were positioned in the buffer above the tissue construct and Au working electrodes to enable TEER measurement using electrochemical impedance spectroscopy. Impedance could be monitored for up to 2 weeks using a Gamry potentiostat (Gamry, Warminster, PA).