Figure 2.

Validation of microarray data. Inactivation of p53 by the GSE56 peptide (GSE) or shRNA (p53i) in three different human primary fibroblasts delays replicative senescence and attenuates the repression of miRs and their hosts upon senescence. (A) Growth curves for the human primary fibroblasts WI-38 and IMR90 and for the prostate cancer-associated fibroblasts (CAFs) PF179. PDLs, population doublings. (B) QRT–PCR for miR-106b and miR-17-5p, and their host transcripts MCM7 and c13orf25, respectively, in early passage (Young) versus late passage (Old) fibroblasts. Data are represented as mean±s.d.