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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Eur J Cell Biol. 2008 Apr 23;87(8-9):721–734. doi: 10.1016/j.ejcb.2008.02.011

Fig. 4. Ras transformation reduces Rsu-1 colocalization with ILK at focal adhesions.

Fig. 4

E6/7 astrocytes (A) and E6/7 astrocytes transformed with activated Ras (E6/7/Ras) (B–D) were plated on fibronectin-coated coverslips and assayed by immunofluorescence using anti-amino-terminal Rsu-1 and anti-ILK antibodies. Nuclei were counterstained with Topro-3 (to exclude cells with aberrant or multiple nuclei.). Colocalizations are highlighted in circles. Colocalization at focal adhesions is reduced in E6/7/Ras (B). Inhibition of ERK activation (20 µM U0126 for 24 h prior to plating) partially restores Rsu-1 and ILK colocalization at focal adhesions (C). (D) Solvent control (DMSO). Bars: 10 µm (A–D).